※ MiCroKiTS INTRODUCTION:
During cell cycle, numerous proteins organize protein super-complexes at a number of distinct regions of centrosome (spindle pole in budding yeast), kinetochore/centromere, cleavage furrow/midbody (related or homolog structures in plants and budding yeast called as phragmoplast and bud neck, respectively), telomere and spindle spatially and temporally. These super super-complexes play important roles in various biological processes. In this work, we have collected all proteins identified to be localized on kinetochore, centrosome, midbody, telomere and spindle from two fungi (S. cerevisiae and S. pombe) and five animals, including C. elegans, D. melanogaster, X. laevis, M. musculus and H. sapiens based on the rationale of "Seeing is believing" (Bloom K et al., 2005). Through ortholog searches, the proteins potentially localized at these super-complexes were detected in 143 eukaryotes. Then the integrated and searchable database MiCroKiTS - Midbody, Centrosome, Kinetochore, Telomere and Spindle has been established. Currently, the MiCroKiTS 4.0 database was updated on June 27, 2014, containing 81,226 unique protein entries. The database will be updated routinely as new microkits proteins are reported.
For publication of results please cite the following articles:
MiCroKiTS 4.0: an updated database of midbody, centrosome and kinetochore, telomere and spindle.
Zhengnan Huang, Zexian Liu, Yongbo Wang, Tianshun Gao, Zhicheng Pan, Jian Ren and Yu Xue. Submitted
MiCroKit 3.0: an integrated database of midbody, centrosome and kinetochore.
Jian Ren, Zexian Liu, Xinjiao Gao, Changjiang Jin, Mingliang Ye, Hanfa Zou, Longping Wen, Zhaolei Zhang, Yu Xue and Xuebiao Yao. Nucleic Acids Research. 2010;38:D155-D160