MiCroKit 3.0 - Midbody, Centrosome & Kinetochore Database
Tag Content
MiCroKit ID
MCK-SC-00096 
UniProt Accession
Theoretical PI
9.72 
Molecular Weight
42945.90 
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
Spindle assembly checkpoint kinase 
Protein Synonyms/Alias
Aurora kinase; Increase-in-ploidy protein 1 
Gene Name
IPL1 
Gene Synonyms/Alias
YPL209C 
Created Date
02-Jun-2006 
Localization
Kinetochore 
Organism
Saccharomyces cerevisiae (Baker's yeast) 
NCBI Taxa ID
NCBI_TaxID=4932 
Reference
[1] Structure, function, and regulation of budding yeast kinetochores.
McAinsh AD, Tytell JD, Sorger PK.
Annu Rev Cell Dev Biol. 2003;19:519-39.[PMID: 14570580]
[2] The kinetochore protein Ndc10p is required for spindle stability and cytokinesis in yeast.
Bouck DC, Bloom KS.
Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5408-13. Epub 2005 Apr 4.[PMID: 15809434] 
Functional Description
Required for high-fidelity chromosome segregation during the later part of each cell cycle. Acts in opposition to the phosphatase PP1. Has a role in attaching the kinetochores to the microtubules and ensuring that sister kinetochores connect to opposite poles. The promotion of bi-orientation is achieved by selectively detaching kinetochore-microtubule attachments that are not under tension. Phosphorylates histone H3 to form H3S10ph during mitosis and meiosis. 
Sequence Annotation
 
Keyword
ATP-binding; Cell cycle; Chromosome partition; Complete proteome; Kinase; Kinetochore; Nucleotide-binding; Nucleus; Phosphoprotein; Serine/threonine-protein kinase; Transferase.  
Sequence Source
UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Nucleotide Length
1104 bp 
Nucleotide Sequence
atgcaacgca atagtttagt aaatatcaaa ctaaacgcta attcgccatc gaaaaagacc 60
acaacaagac caaatacgtc caggatcaat aaaccatgga gaatatccca ttcgccgcag 120
caaagaaacc cgaattcaaa aataccttca cctgtaagag aaaaattgaa cagattacct 180
gtaaacaata agaagttttt ggatatggaa agctccaaaa ttccatcacc tataaggaaa 240
gcgacttctt ccaaaatgat acacgaaaat aagaagctac ctaaatttaa atccctatca 300
ctcgatgact ttgaactggg gaagaaatta ggaaagggta aattcggtaa agtttattgc 360
gttcggcaca ggagtacagg atatatttgc gcactgaaag taatggagaa ggaagaaata 420
ataaagtata atttacagaa acaattcaga agggaggtag aaatacaaac atcgctaaat 480
catccgaatc taactaaatc atacggctat tttcatgatg aaaaaagagt gtacctgcta 540
atggaatact tagtcaatgg ggaaatgtat aaactattga ggttacacgg acccttcaac 600
gatattttag catcagatta tatttatcaa attgccaatg ccctagatta tatgcataaa 660
aagaatatta ttcatagaga tattaaacct gaaaatatac taatagggtt caataatgtc 720
attaagttaa cggacttcgg atggagtata ataaatccgc cagaaaatag aaggaaaact 780
gtctgtggga caattgacta cctttctcca gaaatggtgg agtcaaggga atatgatcac 840
actatagatg catgggctct tggcgtcctg gcgtttgaac tactgaccgg tgcccctccg 900
ttcgaagaag aaatgaaaga tactacatat aaaaggatag cagcactgga tatcaaaatg 960
cccagtaaca tttctcagga tgcgcaagat ttaatactta aactactaaa atacgacccc 1020
aaagatagaa tgcgccttgg agacgtaaaa atgcatcctt ggatactaag aaacaagccc 1080
ttttgggaaa ataagcggtt atag 1104 
Protein Length
367 AA 
Protein Sequence
MQRNSLVNIK LNANSPSKKT TTRPNTSRIN KPWRISHSPQ QRNPNSKIPS PVREKLNRLP 60
VNNKKFLDME SSKIPSPIRK ATSSKMIHEN KKLPKFKSLS LDDFELGKKL GKGKFGKVYC 120
VRHRSTGYIC ALKVMEKEEI IKYNLQKQFR REVEIQTSLN HPNLTKSYGY FHDEKRVYLL 180
MEYLVNGEMY KLLRLHGPFN DILASDYIYQ IANALDYMHK KNIIHRDIKP ENILIGFNNV 240
IKLTDFGWSI INPPENRRKT VCGTIDYLSP EMVESREYDH TIDAWALGVL AFELLTGAPP 300
FEEEMKDTTY KRIAALDIKM PSNISQDAQD LILKLLKYDP KDRMRLGDVK MHPWILRNKP 360
FWENKRL 367 
Gene Ontology
GO:0032133; C:chromosome passenger complex; IDA:SGD.
GO:0000778; C:condensed nuclear chromosome kinetochore; IDA:SGD.
GO:0005828; C:kinetochore microtubule; IDA:SGD.
GO:0051233; C:spindle midzone; IDA:SGD.
GO:0005524; F:ATP binding; IEA:InterPro.
GO:0005515; F:protein binding; IPI:IntAct.
GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.
GO:0008608; P:attachment of spindle microtubules to kinet...; IMP:SGD.
GO:0045144; P:meiotic sister chromatid segregation; IMP:SGD.
GO:0051228; P:mitotic spindle disassembly; IMP:SGD.
GO:0006468; P:protein amino acid phosphorylation; IEA:InterPro.
GO:0032465; P:regulation of cytokinesis; IMP:SGD.
GO:0031577; P:spindle checkpoint; IMP:SGD. 
Interpro
InterPro; IPR000719; Prot_kinase_core.
InterPro; IPR017441; Protein_kinase_ATP_BS.
InterPro; IPR017442; Se/Thr_pkinase-rel.
InterPro; IPR008271; Ser_thr_pkin_AS.
InterPro; IPR002290; Ser_thr_pkinase. 
Pfam
Pfam; PF00069; Pkinase; 1. 
SMART
SMART; SM00220; S_TKc; 1. 
PROSITE
PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PROSITE; PS00108; PROTEIN_KINASE_ST; 1. 
PRINTS
 
Orthology
   Species        Gene Name        Accession   Score  E-Value  Ident.  Pos.
 S. pombe         ark1           MCK-SP-00050  295.0  9e-081    45%    68%
 C. elegans       air-1          MCK-CE-00007  225.0  4e-059    41%    63%
 C. elegans       air-2          MCK-CE-00069  244.0  8e-065    44%    63%
 D. melanogaster  aur            MCK-DM-00062  236.0  3e-062    40%    61%
 D. melanogaster  ial            MCK-DM-00026  236.0  3e-062    41%    61%
 X. laevis        aurka          MCK-XL-00020  265.0  2e-071    41%    60%
 X. laevis        Eg2-like          Q91819     263.0  1e-070    43%    63%
 X. laevis        aurkb-A        MCK-XL-00026  259.0  2e-069    42%    64%
 X. laevis        aurkb-A        MCK-XL-00054  259.0  2e-069    42%    64%
 M. musculus      Aurka             P97477     257.0  2e-068    42%    62%
 M. musculus      Aurkc          MCK-MM-00076  255.0  8e-068    46%    64%
 M. musculus      Aurkb          MCK-MM-00056  253.0  3e-067    44%    66%
 H. sapiens       AURKC          MCK-HS-00278  258.0  1e-068    45%    64%
 H. sapiens       AURKA          MCK-HS-00200  261.0  2e-069    42%    61%
 H. sapiens       AURKB          MCK-HS-00359  255.0  1e-067    40%    60%